[NMRFAM enews] Call for Proposals at NMRFAM

Milo Westler milo at nmrfam.wisc.edu
Thu Nov 21 15:25:24 CST 2013

Call for Proposals

National Magnetic Resonance Facility at Madison (NMRFAM)

NMRFAM will submit a competitive renewal application in mid-January 2014.

We are soliciting proposals from prospective collaborators and facility
users for inclusion in the proposal.

Highest priorities are challenging biomedically-related projects that will
continue to drive the development of technology at NMRFAM. Examples of such
projects are:

• Structure determination and structure-function studies of small proteins
(up to 24 kDa) that utilize our evolving approach for automated NMR data
collection and analysis

• Investigations of protein-protein and protein-peptide complexes and
studies of molecular machines by NMR spectroscopy and/or small-angle X-ray

• Structure-function studies:

o Protein-nucleic acid complexes

o Membrane proteins

o RNA molecules

o Enzyme mechanisms

o Iron-sulfur proteins and their complexes

• Determination of the structures of natural products:NMRFAM’s LC-MS system
with solid-phase

extraction can be used to collect samples as a function of mass for NMR

• Metabolomics studies, particularly ones of a translational nature

• High-throughput screening of proteins and other macromolecules for
interactions with metabolites and other small molecules

Proposals are also welcome for studies that make use of NMRFAM’s current

NMRFAM houses NMR spectrometers with solution-state 1H, 13C, 15N, 31P, and
2H cryogenic probe capability at 900 MHz, 800 MHz, 750 MHz, 600 MHz, or 500
MHz. A 500 MHz NMR spectrometer is equipped for solid state NMR experiments
with cross-polarization / magic angle spinning spectroscopy (CP-MAS) with a
3.2 mm 1H{13C,15N} triple resonance probe or a 4.0 mm
also a 4mm high-resolution magic angle spinning (HR-MAS) probe.

NMR data can be collected at all available field strengths as a function
of: temperature, illumination, or hydrostatic pressure.

NMRFAM has a Bruker Nanostar small-angle X-ray scattering (SAXS) system
with variable temperature capability.

Additional information is provided on the NMRFAM website:

Our goal is to add a spectrometer operating at 1.2 GHz in the coming 5-year

Please contact John Markley if you have any questions:
markley at nmrfam.wisc.edu

Basic information required (kindly respond by 12/20/2013):

(1) Name of the PI:

(2) Institution, Department of the PI:

(3) Project title:

(4) External funding status of the project: source of funds, grant number,
starting date – end date

Description of the project

Significance / impact

How it will make use of NMRFAM technology and/or expertise of NMRFAM staff

1H{X} broad-band magic angle spinning probe. There is

Page limit: 2-6 pages. Figures are always helpful

-- Milo
National Magnetic Resonance Facility at Madison
      An NIH-Supported Resource Center

W. Milo Westler, Ph.D.

NMRFAM Director
Senior Scientist
Adjunct Professor
Department of Biochemistry
University of Wisconsin-Madison
433 Babcock Drive
Madison, WI USA 53706-1544
EMAIL: milo at nmrfam.wisc.edu
PHONE: (608)-263-9599
FAX: (608)-263-1722
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