[NMRFAM enews] Protein Structure Determination Workshop - 2013

Rita Hannah rhannah at wisc.edu
Thu May 2 14:08:18 CDT 2013


I would like to remind you all of the following workshop for anyone
interested in learning the basics for protein structure determination with
the continued support from our NMR staff post-workshop. I would like your
help in dissemination of this information so that anyone interested in it
can register for the workshop:

NMRFAM is hosting its annual Protein Determination Workshop from June 9th
until June 14th, 2013. Registration will close May 28th. 

 

2013 NMRFAM Protein Structure Determination Workshop

Date: June 9th - June 14th, 2013

Location: Department of Biochemistry, University of Wisconsin-Madison, 433
Babcock Drive, Madison, WI 53706-1544.

Purpose: This introductory, hands-on workshop will cover the basic steps
involved in solving a protein structure by NMR and give participants an
opportunity to practice these steps in hands-on sessions led by the NMRFAM
staff. Using triple-resonance NMR data collected at NMRFAM on labeled
ubiquitin, the staff will guide participants through the following steps of
protein structure determination:

1) Description of data collection (raw data will be provided) 

2) Peak picking 

3) Backbone Assignment 

4) Side chain Assignment 

5) TOCSY and NOE Assignments 

6) Automated assignments 

7) Three dimensional structure determination and refinement

8) Deposition of the data in BioMagResBank 

9) Appendix Session : Data Processing

Due to the complexity of solving a protein structure by NMR, this workshop
is intended to give participants a broad overview of the process so they can
begin their own projects. It is expected that participants will require
further assistance to solve a structure on their own. Participants are
invited to contact NMRFAM staff in the future with any questions or problems
they may have.

Format: Each section will begin with a short lecture/demonstration followed
by a hands-on session in which participants will be given time to perform
each step of protein structure determination using data provided by NMRFAM.
The staff will be available during the sessions to answer questions and
assist participants.

Participant requirements: Workshop participants are not expected to have a
prior experience with basic steps of NMR protein structure determination.
The workshop will cover the entire process and each session will build upon
the previous sessions. Due to the complexity of protein structure
determination, participants will be given the data necessary to complete
each session. For example, processed data will be provided for the peak
picking session and fully peak-picked data will be provided for the backbone
and side-chain assignment sessions. Each participant is required to bring
their own laptop computer to the workshop. The laptop needs either wireless
capability or a wired ethernet port. We also recommend that participants
bring a mouse for their laptop.

Registration: The fee for the workshop is $550.00 and includes all materials
necessary to participate in the workshop, a reception on June 9th, a
barbecue dinner on June 13th, with morning and afternoon refreshments. The
last date for registration with University/Campus housing is May 23rd, 2013,
and housing at Best Western InnTowner hotel is available until May 8th,
2013. There will be no guaranteed housing after these dates. You will need
to make your own arrangements for housing and parking if required for the
duration of the workshop after May 23rd, 2013. Registration for the workshop
will continue to be open until May 27th, 2013.
<http://www.nmrfam.wisc.edu/Documents/13_NMR_registration_form.pdf> Click
here for registration form.  Send completed registration form to
<mailto:rhannah at nmrfam.wisc.edu> Dr. Rita Hannah

Lodging: There are 2 lodging options available and they are as follows:

We have a block of rooms that you can call (608) 233-8778 to reserve  at the
<http://www.inntowner.com/index.cfm> Best Western InnTowner Hotel ,  located
at 2424 University Ave Madison, WI 53726. It is ~1.2mile away from the
workshop and on a bus route. The rooms per night are $77.00 /$99.00 for
single /double occupancy and includes complimentary underground parking with
free WIFI. Please ask for a room under the Group Name: NMRFAM. Rooms will be
held until May 8th, 2013. Please note to obtain these rates you will need to
show your State University ID.

We have also reserved a block of rooms for the NMRFAM Workshop on campus at
the Vel Phillips (formerly named Friedrick) Residence Hall. The rate per
night is $57.10 per person for single occupancy and $36.25 per person for
double occupancy. The online reservation site for Phillips Residence Hall
can be done through  <http://www.housing.wisc.edu/summerprograms> Housing or
use the more
<https://regstg.com/Registration/Introduction.aspx?rid=f6e77d62-b2f9-4172-ab
7c-fc5d4e42eca5> direct link. It provides important information regarding
accommodations and services. Please contact the conference office at (608)
262-5576 or  <mailto:Sharon.Seagren at housing.wisc.edu> Sharon Seagren or
<mailto:Tom.Krueger at housing.wisc.edu> Tom Krueger at (608) 262-3024 to make
your reservations. Rooms will be held until May 23rd, 2013.

Please be aware that the Vel Phillips Residence Hall is a University
dormitory and parking is not included at the Vel Phillips residence Hall. If
you need parking on campus, please go to
<http://transportation.wisc.edu/parking/visitor.aspx> UW Transportation
Services or e-mail  <mailto:visitor-parking at fpm.wisc.edu> Parking to arrange
for a parking permit. Alternatively, you can call their office at
608-262-8683.

Please note that registration payment can be made using our online payment
site or by check. Do not combine registration and lodging payment together.
You must pay for your housing directly when making a reservation to Housing
or to the Union South on your arrival.

Please contact  <mailto:rhannah at nmrfam.wisc.edu> Dr. Rita Hannah or phone
(608) 262-3173 if you have questions.

 

Thank you.

Sincerely,

Rita

 

Rita Hannah, Ph.D.

NMRFAM

University of Wisconsin - Madison

Madison, WI - 53706

e-mail:  <mailto:rhannah at nmrfam.wisc.edu> rhannah at nmrfam.wisc.edu

Phone; (608) 262-3173

FAX: (608) 262-3759

 

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